Rhea
(Jung-Fen) Chiang
B.S.
Integrative Physiology of Aging Laboratory, Carlson
1B06, 354 UCB
Department
of Integrative Physiology
University of Colorado at
Boulder
Boulder,
CO 80309-0354, USA
Phone:
(303) 735-4799
FAX:
(303) 492-6778
Educational History
- Metropolitan State College of Denver January 2001 - August 2003, B.S. in Chemistry
- University of Colorado at Boulder August 1999 - August 2000
- Red Rocks Community College August 1998 - May 1999
Current Laboratory Responsibilities
- Immunofluorescent staining and analysis of endothelial cells
- Protein expression analysis on peripherral blood mononuclear cells
- Management of wet lab
- Maintenance of frozen human samples
- Training of new students and personnel
- Procurement of lab supplies
- Weekly experiment schedule update
Techniques and Skills
-
Molecular Cellular Skills/Wet Lab
- Endothelial
cell collection and processing
- Immunofluorescent staining
- Immunofluorescent protein expression analysis
- Mononuclear cell isolation
- DNA extraction
- Ex-vivo whole blood cytokine production
- Western
blot analysis
- Realtime
RT-PCR
- Preparation of auger petri dishes
- Preparation of buffer solution
- In-vitro whole vessel vascular reactivity
- Mouse
tissue weighing and preserving
-
Physiological Testing Skills
- In-vitro whole vessel vascular reactivity
- Mouse tissue weighing and preserving
-
Software/Computer Skills
- Microsoft Excel
- Microsoft Word
- Internet researching
- On-line purchasing
- Webpage
design
Abstracts
- Donato
A., Chiang R., Pierce G.,
Lawson B. & Seals D. Sedentary aging is assicaited with a
senescent endothelial cell phenotype that is ameliorated by habitual
aerobic exercise. FASAB J. Apr 2009; 23: 965.15
- Pierce G., Donato A., Silver A., Chiang R. & Seals D. Habitual aerobic exercise is associated with reduced endothelial cell oxidative stress, NADPH oxidase and NFkB in middle-aged and older humans. FASAB J. Apr 2009; 23: 955.18
Personal Interests
- Baking
- Watching movies