Methods and Logic: Autumn 2005
Mike Klymkowsky & Mike Stowell
Tuesdays, 9:30-10:45AM in A350 MCDB

Topic 6: Chaperones and Genetic Noise - Toby Franks

Sternberg N. 1973. Properties of a mutant of Escherichia coli defective in bacteriophage lambda head formation (groE). I. Initial characterization. J Mol Biol. 1973 May 5;76(1):1-23.

Sternberg N. 1973. J Mol Biol. 1973 May 5;76(1):25-44.
Properties of a mutant of Escherichia coli defective in bacteriophage lambda head formation (groE). II. The propagation of phage lambda.

Queitsch C, Sangster TA, Lindquist S.  Nature. 2002 Jun 6;417(6889):598-9. Hsp90 as a capacitor of phenotypic variation.

Sollars V, Lu X, Xiao L, Wang X, Garfinkel MD, Ruden DM. Nat Genet. 2003 Jan;33(1):70-4.Evidence for an epigenetic mechanism by which Hsp90 acts as a capacitor for morphological evolution.

Topic 6: Neural Noise - Chris Wrobel (part 1) & Xin Geng(part 2)

HODGKIN AL, HUXLEY AF, KATZ B.  J Physiol. 1952 Apr;116(4):424-48. Measurement of current-voltage relations in the membrane of the giant axon of Loligo.

HODGKIN AL, HUXLEY AF.  J Physiol. 1952 Apr;116(4):449-72.Currents carried by sodium and potassium ions through the membrane of the giant axon of Loligo.

HODGKIN AL, HUXLEY AF.
J Physiol. 1952 Apr;116(4):473-96.The components of membrane conductance in the giant axon of Loligo.

HODGKIN AL, HUXLEY AF.
J Physiol. 1952 Apr;116(4):497-506. The dual effect of membrane potential on sodium conductance in the giant axon of Loligo.

Katz B, Miledi R.
Proc R Soc Lond B Biol Sci. 1967 Jan 31;167(6):23-38. The release of acetylcholine from nerve endings by graded electric pulses.

Katz B, Miledi R.
Nature. 1970 Jun 6;226(5249):962-3. Membrane noise produced by acetylcholine.

Neher E, Sakmann B. Nature. 1976 Apr 29;260(5554):799-802. Single-channel currents recorded from membrane of denervated frog muscle fibres

Topic 5: Gene expression - Ka Lin Swain (part 1) & Nathan Gomes (part 2)

Berg, H.C. & D.A Brown. 1972. Chemotaxis in E. coli analyzed by three-dimensional tracking. Nature 239:500-504. (background).

Spudich, J.L. & D.E. Koshland, Jr. 1976. Non-genetic individuality: chance in the single cell.  Nature 262: 467-471.

MacAdams, H. & A. Arkin. 1997. Stochastic mechanisms in gene expression. Proc. Natl. Acad. Sci. USA 94:814-9.

Ozbudak, E.M., M. Thattai, I. Kurster, A.D. Grossman & A.van Oudenaarden. 2002. Regulation of noise in the expression of a single gene. Nature Genetic 31:69-73.

Blake, W.J., M. Kaern, C.R. Canter & J.J. Collins. 2003. Noise in eukaryotic gene expression. Nature 422:633-637.

Fraser, H.B., A.E. Hirsh, G. Giaever, J. Kumm & M.B. Eisen. 2004. Noise minimization in eukaryotic gene expression. PLoS Biol. 2:e167.

Topic 4: DNA Structure - Megan Wemmer

Chargaff,  E. 1950. Chemical specificity of nucleic acids and mechanism of their enzymic degradation. Experientia 6:201-209.  Background on Chargaff 

Franklin, R. Gosling. 1953. Molecular configuration in sodium thymonucleate. Nature 171:740-741.

Watson, J. & F. Crick. 1953. A structure for deoxyribose nucleic acid. Nature 171: 737-738. 

Allemand JF, Bensimon D, Lavery R, Croquette V. 1998. Stretched and overwound DNA forms a Pauling-like structure with exposed bases. Proc Natl Acad Sci U S A. 199895:14152-


Topic 3: Genetic Material - Ryan Takeshita

Griffith, F. 1928. "The significance of Pneumococcal types." J. Hygiene 27:141-144. 

Avery, O.T., C.M. MacLeod & M. McCarty. 1944. Studies on the Chemical Nature of the Substance Inducing Transformation of Pneumococcal Types; Induction of Transformation by a Desoxyribonucleic Acid Fraction Isolated From Pneumococcus Type III. J. Exp. Med. 79:137-158.

Hershey & Chase. Independent functions of viral protein and nucleic acid in growth of bacteriophage. J. Gen. Physiol. 36:39-56.

Background on the discovery of DNA (as genetic material)


Topic 2: Plasma membrane - Andrew Troy

Gorter, E. & F. Grendel. 1925. On bimolecular layers of lipids on the chromocytes off the blood. J. Exp. Med. 41: 439-443.

Frye, L.D. & M. Edidin. 1970. The rapid intermixing of cell surface antigens after formation of mouse-human heterokaryons. J. Cell Science 7: 319-325.

Singer, S.J. 2004. Some early history of membrane molecular biology. Annu. Rev. Physiol. 66:1-27

Van Meer, G., E.H. Stelzer, R.W. Wignaendts-van Resandt & K. Simons. 1987. Sorting of sphingolipids in epithelial (Madin-Darby canine kidney) cells. J. Cell Bio. 105: 1623-1635.


Topic 1: Cytoskeleton - Justin Kuczynski

Gorter, E. & F. Grendel. 1925. On bimolecular layers of lipids on the chromocytes off the blood. J. Exp. Med. 41: 439-443.

Frye, L.D. & M. Edidin. 1970. The rapid intermixing of cell surface antigens after formation of mouse-human heterokaryons. J. Cell Science 7: 319-325.

Singer, S.J. 2004. Some early history of membrane molecular biology. Annu. Rev. Physiol. 66:1-27

Van Meer, G., E.H. Stelzer, R.W. Wignaendts-van Resandt & K. Simons. 1987. Sorting of sphingolipids in epithelial (Madin-Darby canine kidney) cells. J. Cell Bio. 105: 1623-1635.


Updated: 9 September '05