Lisa
A. Lesniewski, Ph.D.
University of Colorado at
Boulder
Department of Integrative Physiology
354
UCB
Boulder, CO
80309
Phone: 303-492-3106
FAX: 303-492-6778
E-mail: Lisa.Lesniewski@colorado.edu
RESEARCH INTERESTS:
• Determining the
effects of healthy aging on the vascular system
•
Understanding the mechanisms of the beneficial effects of habitual
exercise on the vascular system
•
Understanding the mechanisms of impaired insulin
sensitivity with aging
CURRENT PROJECTS:
• Role of
NFkB/TNF in mediating impaired insulin sensitivity and vascular
endothelial dysfunction in humans
•
Role of oxidative stress, inflammation and apoptosis in vascular
endothelial dysfunction in the aged mouse
•
Mechanisms of increased arterial stiffness with aging in the mouse
•
Role of oxidative stress and inflammation in impaired insulin
sensitivity with aging
•
Mechanisms of improved endothelial function in the aged mouse following
exercise training
EDUCATION:
Date
Institution
Degree
Field
1996
Ohio State University
B.S.
Exercise
Physiology
1999
Kent State
University
M.A.
Exercise Physiology
2004
Texas A&M
University
Ph.D.
Exercise
Physiology
EMPLOYMENT:
Date
Position-Institution-Advisor/Mentor
1997-1999
Graduate Teaching Assistant-Kent State University- Wayne Sinning
1999-2004
Graduate Research
Assistant-Texas A&M University- Michael Delp
2004-2006
Postdoctoral
Fellow-University of California at San Diego- Jerrold Olefsky
2006-present
Senior Research
Associate-University of Colorado at Boulder- Douglas Seals
PROFESSIONAL SOCIETIES:
2000-present American
Diabetes Association
2002-present American
Physiological Society
2003-present American Heart Association
TEACHING EXPERIENCE:
1997-1999
Graduate Teaching Assistant: Lifetime Fitness and Activity Courses:
Kent State University
2006,2008
Co-Instructor: IPHY 6010 Physiology of Aging: University of Colorado at
Boulder
2007
Co-instructor: MCDB 4100 Role of Science in Medicine (Lecture: Vascular
measurements and
consequences of vascular aging.) University of Colorado at Boulder
HONORS AND AWARDS:
1996
Undergraduate
Honors Research Scholarship, Ohio State University
1998
Student
Research Development Award, Mid-West Chapter of the American
College of Sports Medicine
1999-2000 University Merit Scholarship, Texas
A&M University
2001
Student Research
Development Award, Texas Chapter of the American College of
Sports Medicine
2000
Student
Manuscript Award, Texas Chapter of the American College of
Sports Medicine
2004
Student
Manuscript Award, Texas Chapter of the American College of
Sports Medicine
2004-2005 Position on Institutional NRSA-T32
HD007203-24, Training in Reproductive Sciences
2007-2008 Position on Institutional NRSA-T32
AG000279-06A1, University of Colorado Aging Training Grant
2007-2009 NIH Loan Repayment Grant,
Inflammation, Vascular Function, and Insulin Sensitivity in Older Adults
2008
Recognition Award for Meritorious
Research by a Young Investigator, Endocrinology and Metabolism
Section-American Physiological Society
GRANT FUNDING:
2005-2006 Individual
NRSA-F32 DK072815-01: Role of CAP in In Vivo Insulin Action.
2009 Mentored Research Scientist Development
Award-KO1 AG033196-01: Aging, Western Diet and Physiological
Dysfunction: Exercise and Inflammation
AD HOC REVIEWER:
American Journal of Physiology (Heart and Circulation)
Journal of Applied Physiology
Experimental Gerontology
PRESENTATIONS:
University of Colorado at Boulder, Department of Integrative
Physiology Colloquium, Boulder, CO, March 2009, “Does aging exacerbate the metabolic and
vascular consequences of a western diet?”
Colorado State University,
Department of Exercise Science Colloquium, Fort Collins CO, March 2009,
“Does aging exacerbate the metabolic
and vascular consequences of a western diet?”
Keystone Symposia: Dissecting the
Vasculature: Function, Molecular Mechanisms and Malfunction,
Vancouver, British Columbia, Canada, February 2009, “Age-associated endothelial dysfunction is
associated with IKKβ-mediated reductions in FoxO3a activity and MnSOD
expression”
University of New South Wales,
Sydney, Australia, December 2008, “Effects
of aging and exercise on western diet-associated metabolic and vascular
dysfunction”
Mayo Clinic, Scottsdale AZ,
October 2008, “Effects of aging on
western diet-associated metabolic and vascular dysfunction”
Federation for the American Societies
of Experimental Biology, San Diego, CA, April 2008, “Salsalate lowers fasting blood glucose
concentrations without improving insulin resistance in non-diabetic
older adults with the metabolic syndrome”
Keystone Symposia: Adipogenesis,
Obesity and Inflammation, Vancouver, British Columbia, Canada,
January 2006, “Macrophage-specific
CAP gene deletion protects against high fat diet induced insulin
resistance”
American Diabetes
Association-Scientific Sessions, San Diego, CA, June 2005, “CAP deletion protects against high fat
diet-induced insulin resistance”
American College of Sports
Medicine-Texas Chapter Annual Meeting, Tyler, TX, February 2004,
“Endothelial dysfunction accompanies
the onset of diabetes and
hypertension in the Zucker diabetic
fatty rat”
American Heart Association Scientific
Sessions, Orlando, FL, November 2003, “Enhanced vasoconstrictor responsiveness
precedes endothelial dysfunction in skeletal muscle arterioles in the
progression of type II diabetes”
Federation for the American Societies
of Experimental Biology Annual Meeting, San Diego, CA, April
2003, “Vasoconstrictor
responsiveness in isolated skeletal muscle arterioles from prediabetic
Zucker diabetic fatty rats”
American College of Sports
Medicine-Texas Chapter Annual Meeting, Fort Worth, TX, February
2001, “Evidence for EC uncoupling in
fast twitch skeletal muscle
from mice with streptozotocin-induced
diabetes”
BIBLIOGRAPHY:
Refereed Journal Articles:
Donato AJ, Pierce GL, Lesniewski LA,
Seals DR. Role of NFκB in Age-Related Vascular Endothelial Dysfunction
in Humans. AGING (In Press).
Donato AJ, Papadopoulos A, Dominguez JM, Lesniewski LA and Delp MD. Aging,
exercise training and advance glycation endproducts: Effects on
arterial structure and stiffness. Am J Physiol Heart Circ. In
Review.
Durrant JR, Connell ML, Folian BJ, Russell MJ, Donato AJ, Seals DR
and Lesniewski LA.
Voluntary wheel running restores endothelial function in conduit
arteries of old mice: Direct evidence for reduced oxidative stress,
increased superoxide dismutase activity and down-regulation of NADPH
oxidase. J. Physiol. Jul 2009; 587: 3271-3285.
Pierce GL, Lesniewski LA,
Lawson BR, Beske SD and Seals DR. Nuclear factor κ B activation
contributes to vascular endothelial dysfunction via oxidative stress in
overweight/obese middle-aged and older humans. Circulation. Mar 2009;
119: 1284-1292.
Lesniewski LA, Connell ML,
Durrant JR, Folian BJ, Anderson MC, Donato AJ and Seals DR. B6D2F1 mice
are a suitable model of oxidative stress-mediated impaired
endothelium-dependent dilation with aging. J Gerontol. A Biol. Sci.
Med. Sci., Jan 2009; 64A: 9-20.
Lesniewski LA, Donato AJ,
Behnke BJ, Woodman CR, Laughlin MH, Ray CR and Delp MD. Enhanced
vasoconstrictor reactivity of skeletal muscle arterioles in the Zucker
diabetic fatty rat occurs prior to overt diabetes and hypertension.
Amer J Physiol Heart Circ Physiol, Apr 2008; 294: H1840-50.
Lesniewski LA, SE Hosch, JG
Neels, C de Luca, M Pashmforoush, CN Lumeng, SH Chiang, M Scadeng, AR
Saltiel, and JM Olefsky. Bone marrow specific CAP gene deletion
protects against the development of high fat diet-induced insulin
resistance. Nature Med., Apr 2007; 13: 455-62.
Donato AJ, LA Lesniewski, and
MD Delp. Aging and exercise training alter adrenergic vasomotor
responses of rat skeletal muscle arterioles. J. Physiol., Feb 2007;
579:115-125.
Behnke BJ, RD Prisby, LA Lesniewski,
AJ Donato, HM Olin, and MD Delp. Effects of aging on physical activity
and vascular morphology and shear-stress in rat skeletal muscle. J.
Physiol., Sep 2006: 575: 617-626.
Donato AJ, Lesniewski LA, and
Delp MD. The effects of aging and exercise training on endothelin-1
sensitivity in rat skeletal muscle arterioles. Cardiovas. Res., May
2005; 66: 393-401.
Wilkerson MK, LA Lesniewski,
EM Golding, RM Bryan, A Amin, E Wilson, and MD Delp. Simulated
microgravity enhances cerebral artery vasoconstriction and vascular
resistance through an endothelial nitric oxide mechanism. Am. J.
Physiol. Heart Circ. Physiol., Apr 2005; 288: H1652-H1661.
Lesniewski LA, TA Miller, and
RB Armstrong. Evidence for EC uncoupling in fast twitch skeletal muscle
from mice with streptozotocin-induced diabetes. Muscle & Nerve. Oct
2003; 28: 493-500.
Muller-Delp JM, SA Spier, MW Ramsey,,
LA Lesniewski, A Papadopoulos, JD Humphrey, and MD Delp. Effects
of aging on vasoconstrictor and mehanical properties of rat skeletal
muscle arterioles. Am. J. Physiol. Heart Circ., May 2002; 282:
H1843-H1845.
Miller TA, LA Lesniewski, JM
Muller-Delp, AK Majors, D Scalise, and MD Delp. Hindlimb unloaing
induces a collagen isoform shift in the soleus muscle of the rat.
Am. J. Physiol. Reg. Int. Comp., Nov 2001; 281: R1710-R1717.
Selected Abstracts:
Russell MJ, Folian BJ, Durrant JR, Connell ML, Seals DR
and Lesniewski LA. Aging
increases susceptibility to western diet-induced glucose intolerance
and insulin resistance in mice. FASEB J, Apr 2009; 23: 990.18.
Rippe C, Lesniewski LA, Donato
AJ and Seals DR. Short-term Caloric Restriction Improves Glucose
Tolerance in Older Mice Independent of Changes in SIRT-1. FASEB J, Apr
2009; 23: 990.33.
Walker AE, Pierce GL, Lesniewski LA,
Lawson BR, Magerko KA and Seals DR. Absence of inhibitor of nuclear
factor {kappa} B kinase-mediated suppression of vascular endothelial
function in middle-aged/older adults who exercise. FASEB J, Apr 2009;
23: LB61.
Fleenor BS, Lesniewski LA,
Durrant JR, Connell ML, Donato and Seals DR. Voluntary aerobic exercise
abolishes age-associated arterial stiffening in mice: relation to
collagen subtype expression in the medial and adventitial layers. FASEB
J, Apr 2009; 23: 774.13.
Lawson BR, Magerko KA, Lesniewski LA,
Rippe C, Seals DR and Donato AJ. Aortic microRNA expression is altered
with aging in mice. FASEB J, Apr 2009; 23: 776.1.
Connell ML, Durrant JR, Russell MJ, Donato AJ, Seals DR and Lesniewski LA. Voluntary wheel
running abolishes vascular inflammation and restores endothelial
function in old mice. FASEB J, Apr 2009; 23: 777.6.
Durrant JR, Folian BJ, Connell ML, Russell MJ, Seals DR and Lesniewski LA. Chronic aerobic
exercise opposes age- and high fat diet-associated vascular endothelial
dysfunction: relation to IKKβ and AMPK. FASEB J, Apr 2009; 23: 777.7.
Pierce GL, Lesniewski LA,
Lawson BR, Beske SD and Seals DR. Vascular endothelial nuclear
factor-κB contributes to endothelial dysfunction via oxidative stress
in middle-aged and older overweight/obese humans. Circulation, Oct
2008; 118: S553.
Rippe C, Connell ML, Lesniewski LA,
Donato AJ and Seals DR. Short-term caloric restriction reverses
age-associated vascular endothelial dysfunction in older mice.
Circulation, Oct 2008; 118: S466.
Lesniewski LA, Folian BJ,
Durrant JR, Beske SD, Stob NR, and Seals DR. Salsalate lowers fasting
blood glucose concentrations without improving insulin resistance in
non-diabetic older adults with the metabolic syndrome. FASEB J. Mar
2008; 22: 949.11.
Connell ML, Durrant JR, Folian BJ, Russell MJ, Seals DR and Lesniewski LA. Anti-inflammatory
treatment restores endothelium-dependent dilation in older mice by
increasing nitric oxide bioavailability. FASEB J. Mar 2008; 22: 967.5.
Folian BJ, Durrant JR, Connell ML, Russell MJ, Seals DR and Lesniewski LA. Aging is associated
with increased susceptibility to western diet-induced glucose
intolerance and endothelial dysfunction in mice. FASEB J. Mar 2008; 22:
1226.7.
Durrant JR, Connell ML, Folian BJ, Russell MJ, Seals DR and Lesniewski LA. B6D2F1 mouse model to
study the effects of habitual aerobic exercise on vascular endothelial
dysfunction with aging. FASEB J. Mar 2008; 22: 1235.11.
Lesniewski LA, Connell ML, Folian
BJ, Durrant JR, Donato AJ and Seals DR. Role of superoxide in carotid
artery endothelial dysfunction in the B6D2F1 mouse. FASEB J. Mar 2007;
21: LB94.
