The model system for PNA-FISH: The adjoining image (click to enlarge) illustrates the Heterogeneity of CENP-B repeats in centromeres
Image Details ::
PNA 17-mer: Rho-A TTC GTT GGA AAC GGG A

PNA-FISH: This image illustrates the Species-specific hybridization of CENP-B PNA 17-mers.
Image Details ::
DNA: 5' - TAAGCAACCTTTGCCCT - 3'5' - AAAGCAGATTACGCCCA - 3'
PNA: Flu- TTTCGTCTAATGCGGGT     Flu - TTTCGTCTAATGCGGGT
 

This image shows the Single-base discrimination between two CENP-B PNAs using PNA-FISH on Mus caroli centromeres
 

This image is a diagram showing the base composition and position of 1bp mismatch PNAs
 

PNA-FISH: Discrimination of base mismatch in CENP-B repeats


 

Combined effects mismatched base composition and position of PNA 17-mers on PNA-FISH in Mus musculus

Table showing that Single base discrimination is dependent on base composition and position