Instrumentation

Instrumentation

Center for Environmental Mass Spectrometry

CU Campus



LC/TOF-MS

The LC/TOF-MS instrument (Agilent 6220) is our tool for identification of non-target compounds and unknowns.

It provides accurate masses below 2 ppm error and it has been succesfully used for many of our current projects.

The high resolving power of 4 GHz allows the separation of isobaric interferences from compounds of interest.

Linearity of response is over 2 orders of magnitude.



The ion-trap LC/MS-MS instrument (Agilent XCT Trap) is our tool for confirmation of target compounds and study of fragmentation pathways.

It provides MSn analyses and it has been succesfully applied to the discovery of new metabolites and non-target compounds via fragmentation pathways.

This is an easy instrument to operate and a powerful tool for deciphering fragmentation pathways of emerging contaminants.

LC/MS-MS



LC/QqQ-MS

The LC/QqQ-MS instrument (Agilent 6460) with the JetStream technology is one of the most sensitive triple quadrupoles in the market.

It provides comprehensive screening for a large number of compounds and it gives high confidence by monitoring two or more transitions.

Usual detection limits range from 0.1 ng/L to 10 ng/L for pesticides and from 1 ng/L to 50 ng/L for pharmaceuticals. Linearity of response is over 3 orders of magnitude.

This system is coupled to the new 1290 UHPLC which provides both improved peak resolution and rapid analysis.



The GX-271 ASPEC (Gilson) instrument is our tool for sample preparation. The analytes of interest can be pre-concentrated on Solid-Phase (SPE) extraction cartridges and eluted with small amounts organic solvents, thus reaching very low levels of detection in water samples.

Sample volumes vary from 50 mL to 200 mL for isolation of several organic contaminants.

The easy automation of this system improves the reproducibility and reliability of the analysis.

Gilson SPE



LC/QTOF-MS

Our last acquisition (but not least!), the LC/QTOF-MS instrument (Agilent 6540) with the JetStream technology is one of the most accurate time-of-flight mass spectrometers in the market.

It provides comprehensive screening for a large number of compounds (pesticides and pharmaceuticals) by using extensive accurate mass databases of more than thousands of compounds.

The capability of MS-MS experiments allows to descipher unknown compounds in environmental samples by using unique accurate mass fragmentation information.

This system is also coupled to the new 1290 UHPLC which provides both improved peak resolution and rapid analysis.